Journal: iScience

Article Title: Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron

doi: 10.1016/j.isci.2023.108555

Figure Lengend Snippet:

Article Snippet: Perl’s iron stain kit , American MasterTech , Cat#KTIRO.

Techniques: Recombinant, SYBR Green Assay, Staining, TUNEL Assay, Isolation, cDNA Synthesis, Peptide Fractionation, DC Protein Assay, Quantitative Proteomics, Negative Control, Control, Software

Journal: Biosensors

Article Title: Foreign Body Reaction to Neural Implants: A Comparative Study of Polymer Toxicity and Tissue Response

doi: 10.3390/bios15090599

Figure Lengend Snippet: Morphological changes in brain tissue during the implantation of the phantom polymer samples four weeks post-surgery. ( A , E , I , M , Q , U , Y , AC , AG , AK , AO )—Nissl’s staining showing degeneration of neurons at the site of cortex displacement (yellow arrows highlighting degenerated neurons, red arrows highlighting normal neurons, and magnification 200×). ( B , F , J , N , R , V , Z , AD , AH , AL , AP )—Overview of brain tissue morphology near the implant, hematoxylin and eosin staining, and magnification 400×. ( C , G , K , O , S , W , AA , AE , AI , AM , AQ )—Early formation of fibrotic scar, collagen fibers are stained in blue (Masson’s trichrome staining, blue arrows highlighting collagen layer, and magnification 400×). ( D , H , L , P , T , X , AB , AF , AJ , AN , AR )—Perls’ staining, dying hemosiderin in blue, indicating the traces of hemorrhage occurred at the acute phase along the whole implantation channel (black triangles highlight areas containing hemosiderin, and magnification: ( L )—40×, ( T , AN )—20×, ( other )—30×). Asterisk corresponds to the cavity developed by the implant.

Article Snippet: For the assessment of fibrotic scar formation and visualization of hemosiderin, Mallory’s trichrome stain kit (Biovitrum, Moscow, Russia) and Perls’ Prussian blue staining kit (Biovitrum, Moscow, Russia) were used according to the manufacturer’s instructions.

Techniques: Polymer, Staining